Á¤±âÇмú´ëȸ ¹ßÇ¥ ÃÊ·ÏÀº ÀÎÅͳÝÀ» ÅëÇÑ º» Çùȸ Web site(www.kaobs.or.kr)·Î¸¸ Á¢¼öÇÕ´Ï´Ù.
I. ÃÊ·ÏÀÇ ³»¿ëÀº ÇÑ±Û È¤Àº ¿µ¹®À¸·Î ÀÛ¼ºÇÏ°í, º»¹®ÀÇ ¾çÀº A4 ¿ëÁö·Î ±¹¹®°ú ¿µ¹® ¸ðµÎ 12ÁÙ (ÆùÆ® 12 ±âÁØ), ¿µ¹®ÀÇ °æ¿ì 1200ÀÚ (ȤÀº 200 words) À̳»·Î µÇ°Ô ÇÕ´Ï´Ù.
II ÃÊ·ÏÀÇ ±¸¼º
II-1. Á¦¸ñ(Title)
- (1) Á¦¸ñÀº ÃÊ·ÏÀÇ ³»¿ëÀÌ Àß Ç¥ÇöµÇ¸é¼µµ °£°áÇÏ¿©¾ß ÇÕ´Ï´Ù.
- (2) ¿µ¹®ÀÇ °æ¿ì ÀüÄ¡»ç, °ü»ç¿Í Á¾¡¤¼Ò¸íÀ» Á¦¿ÜÇÑ ¸ðµç ´Ü¾îÀÇ Ã¹ ±ÛÀÚ´Â ´ë¹®ÀÚ·Î ¾²°í, ¾àÀÚÀÇ »ç¿ëÀº °¡±ÞÀû ÇÇÇÕ´Ï´Ù.
II-2. ÀúÀÚ(Author)
- (1) ¿µ¹® ÃÊ·ÏÀÇ °æ¿ì, °¢ ÀúÀÚ¸íÀº full nameÀ¸·Î ¾²µÇ À̸§ ´ÙÀ½¿¡ ¼ºÀÌ ¿Àµµ·Ï Ç¥±âÇÕ´Ï´Ù.
II-3. Authorship (ÀúÀÚ±¸ºÐ)
- (1) ³í¹®¹ßÇ¥ÀÚ´Â ¼º¸íÀÇ ¿ìÃø »ó´Ü¿¡ ¼Ò¹®ÀÚ "p"·Î Ç¥½ÃÇÕ´Ï´Ù.
- (2) ±³½ÅÀúÀÚ´Â ¼º¸íÀÇ ¿ìÃø »ó´Ü¿¡ ¼Ò¹®ÀÚ "c"·Î Ç¥½ÃÇÕ´Ï´Ù.
- (3) ±³½ÅÀúÀÚ°¡ Á÷Á¢ ³í¹®À» ¹ßÇ¥ÇÒ °æ¿ì ¼Ò¹®ÀÚ "d"·Î Ç¥½ÃÇÕ´Ï´Ù.
- (4) ±× ¿ÜÀÇ ÀúÀڴ ǥ½ÃÇÏÁö ¾Ê½À´Ï´Ù.
II-4. ¼Ò¼Ó±â°ü(Affiliation)
- (1) ÇØ´ç ÀúÀÚ¿Í µ¿ÀÏÇÏ°Ô ¹øÈ£¸¦ ÁöÁ¤ÇÏ¿© ¼Ò¼Ó±â°ü, Çаú ¶Ç´Â ¿¬±¸½Ç¸í, µµ½Ã¸í ¹× ¿ìÆí¹øÈ£¸¦ Ç¥±âÇϽʽÿÀ.
- (2) ¼Ò¼Ó±â°ü ¸íÀº ¾àÀÚ¸¦ ¾²Áö ¸¶½Ã°í Àüü À̸§À¸·Î ½áÁֽñ⠹ٶø´Ï´Ù.
(ÀúÀÚ¿Í ¼Ò¼Ó Ç¥±âÀÇ ¿¹),
- Kam Chan Kangp1, Gil-Dong Hong1, and Chun-Hyang Sungc12
1Department of Molecular Biology, Hankook University, Seoul 02850; 2LMO Evalution Laboratory, Korea Research Institute of Bioscience and Biotechnology, Deajeon 34141
II-5. ³»¿ë
- (1) ÃÊ·Ï ¸»¹Ì¿¡ ¿¬±¸¸¦ Áö¿øÇØ ÁØ Áö¿øó¸¦ Ç¥±âÇÒ ¼öµµ ÀÖ½À´Ï´Ù.
ÃÊ·Ï ¿¹
Proteomic Analysis of Encystment Related Proteins in Acanthamoeba castellanii
Kam Chan Kangp, Chun-Hyang Sungc
Department of Molecular Biology, Hankook University, Seoul 02850
Acanthamoeba castellanii have two distinct forms, trophozoite and cyst. When deprived of nutrients or under other stresses, trophozoites undergo a cellular differentiation into cysts. To find the factors mediating encystation and analyze its mechanism, we carried out a proteomic analysis. In total, 48 proteins were identified by MALDI-TOF MS and 11 proteins of these proteins were determined as stage-specific markers. Numerous proteins were differently regulated during encystment, including decrease of cytoskeleton expression, degradation of S-adenosylmethionine synthetase and increase of several defense-related proteins. By 2-D blotting assay, we immunologically identified actins, ubiquitin and HSP70 and found appearance of fragmented actins in mature cyst. Of three immunologically identified proteins, actins were confirmed as A. castellanii actin-1 by MALDI-TOF MS. We concluded that these proteins play an important role in cyst maturation by regulating the ubiquitin-dependent proteolysis system. This study provides some critical clues for understanding cellular differentiation in Acanthamoeba castellanii as a model organism.
* This work was supported by KOSEF grant
¹èÃßÀ¯ÀüüÀÇ ±¸Á¶ ¹× ÁøÈ ¿¬±¸
È«±æµ¿c1, ¹Ú¹®¼öp2
1Á¦ÀÏ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¸í°úÇаú, ¼ö¿ø 16499; 2¿ì¼ö´ëÇб³ ÀÌ°ú´ëÇÐ »ý¹°Çаú, ÆòÅà 17869
¹èÃßÀÇ flowering locus CÀ¯ÀüÀÚ¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Â homologous BAC Ŭ·Ð ´Ù¼¸ °³¸¦ ¼±¹ßÇÏ¿© ¿°±â¼¿ Çص¶ÇÏ°í À̸¦ ¾Ö±âÀå´ë À¯Àüü¿Í ½Éµµ ÀÖ°Ô ºñ±³À¯Àüü ºÐ¼®À» ¼öÇàÇÏ¿© ±¹Á¦ÀûÀ¸·Î Àú¸íÇÑ ÇмúÁöÀÎ Plant Cell¿¡ Åõ°íÇÏ¿© °ÔÀç½ÂÀÎÀ» µæÇÏ¿´´Ù. ¿¬±¸°á°ú¸¦ ¿ä¾àÇÏ¸é ¾Ö±âÀå´ë¿Í ¹èÃß´Â ¾à 1700¸¸ ³â Àü¿¡ ºÐȵǾú´Ù. ±× ÀÌÈÄ ¹èÃß´Â ¾à 1400¸¸ ³â Àü¿¡ 6¹èü°¡ µÇ¾ú°í 80¸¸ ³â Àü¿¡´Â ºÎºÐÀûÀÎ genome duplicationÇö»óÀÌ ÇÑ ¹ø ´õ ¹ß»ýÇÏ¿´´Ù. ÀÌµé °¢°¢ÀÇ »óµ¿ºÎºÐÀº 4±ºµ¥ÀÇ ¿°»öü ºÎÀ§¿¡ Á¸ÀçÇϸç ÀÌµé ºÎÀ§ÀÇ ¼¼¹ÐÇÑ ºÐ¼® °á°ú 6¹èü°¡ µÈ ÀÌÈÄ °¢ ºÎÀ§ÀÇ ¿øÇüÀº ±×´ë·Î À¯ÁöµÇÁö¸¸ ¸¹Àº ºÎÀ§¿¡¼ÀÇ °á½Ç°ú ¼Ò¼öÀÇ ´Ù¸¥ À¯ÀüÀÚ È¤Àº ÀüÀÌÀÎÀÚÀÇ »ðÀÔÀÌ ÀÌ·ç¾îÁ³´Ù. 6¹èü¶õ µ¿ÀÏÇÑ À¯Àüü°¡ ¼¼¹è (genome triplication)·Î Áõ°¡ÇÑ Çö»óÀε¥ ÀÌ Çö»ó¿¡ ÀÇÇØ ¸ðµç À¯ÀüÀÚ´Â ±× ¼ö°¡ 3¹è·Î Áõ°¡ÇÏ¿´´Ù°¡ Áö³ 1400¸¸³âÀÇ À̹èÃ¼È °úÁ¤¿¡¼ À¯ÀüÀûÀ¸·Î ¾ÈÁ¤ÇÑ ±¸Á¶·Î À¯ÀüÀÚÀÇ ¼ö°¡ °á½Ç¿¡ ÀÇÇØ ÀçÁ¶Á¤µÇ¾ú´Ù. ¼¼ °³°¡ µÇ¾ú´ø À¯ÀüÀÚÀÇ ¾à 12%¸¸ÀÌ ¼¼ °³ ¸ðµÎ ¿øÇüÀ» À¯ÁöÇÏ°í ÀÖÀ¸¸ç ´Ù¸¥ 44%´Â homologousÇÑ ¿°»öü ¼¼ ºÎÀ§ Áß ¿°»öü ÇÑ ºÎÀ§¿¡¼ °á½ÇµÇ¾î µÎ°³ÀÇ À¯»çÀ¯ÀüÀÚ (orthologs)¸¸ Á¸ÀçÇÏ°í ³ª¸ÓÁö 44%´Â ¿°»öü ¼¼ ºÎÀ§ Áß µÎ ºÎÀ§¿¡¼ °á½ÇµÇ¾î single copy »óÅ·Πº¹±ÍÇÏ¿´´Ù. À̵é À¯ÀüÀÚ´Â ¸Å¿ì À¯»çÇÏÁö¸¸ ¾à°£ÀÇ Â÷ÀÌ·Î µ¶Æ¯ÇÏ¸é¼ ´Ù¾çÇÑ ±â´ÉÀ» ÇÒ ¼ö ÀÖ´Â À¯ÀüÀÚ±º¿¡ ¼ÓÇÑ´Ù. ¹Ý¸é single copy·Î º¹±ÍÇÑ À¯ÀüÀÚµéÀº ´Ù¼ö°¡ ±× ±â´ÉÀÌ ¾ÆÁ÷ ¹àÇôÁöÁö ¾ÊÀº À¯ÀüÀÚ±ºÀÓÀÌ È®ÀεǾú´Ù. ÀÌ·± °á°úµé·Î º¼¶§ °ÅÀÇ ¿øÇüÀ» À¯ÁöÇÏ°í ÀÖ´Â ¾Ö±âÀå´ëÀÇ À¯Àüü´Â ¾ÆÁ÷µµ ¾ß»ýÀÇ ÀÛÀº ÀâÃÊ·Î ¸Ó¹® ¹Ý¸é ¹èÃß À¯Àüü´Â 6¹èü ÈÄ À̹èÃ¼È µÇ´Â °úÁ¤¿¡¼ ¿ªµ¿ÀûÀÎ º¯È °úÁ¤À» °ÅÃÄ Áö±ÝÀÇ ¾ÈÁ¤ÀûÀ̸ç ÀÌ·Î¿î ±¸Á¶·Î Áö±ÝÀÇ ¹èÃß·Î ÁøȵǾú´Ù°í ¾ê±âÇÒ ¼ö ÀÖ´Ù.